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Codon Optimizer
Convert amino acid sequences to DNA using human codon usage frequencies from the Kazusa database. Codons are selected probabilistically based on their natural frequency in the human genome, which helps reduce repetitive sequences and trends toward natural GC content (~52%).
Input Sequence
Enter your amino acid sequence in FASTA format or as raw text. Valid characters: A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y, * (stop)
About This Tool
Probabilistic Codon Selection: Rather than always selecting the most frequent codon for each amino acid, this tool uses weighted random selection based on natural human codon usage frequencies. This approach:
- Reduces the likelihood of repetitive sequences
- Produces more natural-looking DNA sequences
- Trends toward human-typical GC content (~52%)
- Each optimization run produces a different valid sequence
Codon Usage Data: Frequencies are derived from the Kazusa Codon Usage Database for Homo sapiens (human), which represents codon usage across the entire human genome.